Description
The microtiter plate of the Calretinin ELISA is coated with a capture antibody. The diluted sample is added, and any antigen present binds to capture antibody. After a washing step the detecting antibody (biotinylated anti-calretinin antibody) is added and binds to antigen. After another washing step the enzyme conjugate streptavidin-peroxidase is added and binds to detecting antibody. The following substrate TMB / peroxidase reaction is monitored at 450 nm (reference wavelength at 620 nm)